Influence of isorhamnetin on cultured neonatal rat cardic fibroblast proliferation and collagen synthesis induced by angiotensinII

Objective To study the influence of Isorhamnetin on cultured neonatal rat cardic fibroblast (CFb) proliferation and collagen synthesis induced by angiotensinⅡ. Methods Neonatal rats were treated with angiotensin Ⅱ (AngII) to form cardic fibrosis model. The effects of isorhamnetin on CFb proliferation was measured by thiazolyl blue (MTT) colorimetric assay, synthesis of collagen was measured by 3H- proline incorporation assay. Results Isorhamnetin (10-4- 10-6 mol/L) inhibited angiotensin Ⅱ induced CFb proliferation and collagen synthesis in a dose- dependent manner (P0.01). Conclusion Isorhamnetin inhibited angiotensin Ⅱ induced cardic fibrosis by inhibiting CFb proliferation and collagen synthesis. It suggests that isorhamnetin may play a role in the regression of heart remodeling.