Optimization of Identification Condition of Thin Layer Chromatography for Alpinia Katsumadai Hayata

Abstract objective: To optimize the TLC identification conditions of Alpinia katsumadai Hayata. Methods: Qualitative identification of Alpinia katsumadai Hayata was carried out by TLC, toluene: ethyl acetate: methanol (15: 4: 1) and toluene: ethyl acetate: methanol (14.5: 4.5: 1) were used as developing solvent, then observed under 365nm ultraviolet lamp. Results: The optimized conditions were better than those of TLC in 2020 edition of Chinese Pharmacopoeia. The optimized spots were more clear, the separating capacity was better, and the stability of the conditions was better. After optimization, there were 9 spots in TLC. Conclusion: The method is simple, specific and reproducible, which can provide reference for the quality control of Alpinia katsumadai Hayata.