化学
生物燃料
丁醇
生物过程
食品科学
生物转化
生物转化
代谢工程
原材料
乳糖
生物净化
生物化学
发酵
生物技术
有机化学
生物炼制
生物
乙醇
酶
古生物学
作者
Yuechao Ma,Na Guo,Shangjun Wang,Yifen Wang,Zhihua Jiang,Liang Guo,Wei Luo,Yi Wang
标识
DOI:10.1016/j.biortech.2024.130640
摘要
As a byproduct of dairy production, the disposal of acid whey poses severe environmental challenges. Herein, an innovative solution involving metabolically engineering Clostridium saccharoperbutylacetonicum to convert all carbon sources in acid whey into sustainable biofuels and biochemicals was presented. By introducing several heterologous metabolic pathways relating to metabolisms of lactose, galactose, and lactate, the ultimately optimized strain, LM-09, exhibited exceptional performance by producing 15.1 g/L butanol with a yield of 0.33 g/g and a selectivity of 89.9%. Through further overexpression of alcohol acyl transferase, 2.7 g/L butyl acetate along with 6.4 g/L butanol was generated, resulting in a combined yield of 0.37 g/g. This study achieves the highest reported butanol titer and yield using acid whey as substrate in clostridia and marks pioneering production of esters using acid whey. The findings demonstrate an innovative bioprocess that enhances renewable feedstock biotransformation, thereby promoting economic viability and environmental sustainability of biomanufacturing.
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