ApoB secretion and intracellular lipid content are modulated by ANGPTL3 and PCSK9 in HEPG2 cells

Results: Functional analyses of HepG2-rs879254797 transfected cells showed difference in LDLR expression, and docking molecular analyses show that changing an Asp by a Gly at position 373 decreases the interaction distance between LDLR and ApoB.Besides, it was verified histologically that HepG2-rs879254797 and HepG2-rs750518671 showed unexpected cellular processes, like cell-cell communication, vacuolization and pseudopods.Conclusions: The HepG2-rs879254797 showed less LDLR expression compared to the WT, and also showed important histological result when incubated with LDL, that could suggest a pathological process.