Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

• Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.