Impact of Deep Eutectic Solvents on Extraction of Polyphenols from Grape Seeds and Skin

氯化胆碱 化学 多酚 柠檬酸 苯酚 抗氧化剂 萃取(化学) 氯化物 溶剂 深共晶溶剂 色谱法 食品科学 有机化学 共晶体系 合金
作者
Nevena Dabetić,Vera Todorović,Manuela Panić,Ivana Radojčić Redovniković,Sladjana Sobajic
出处
期刊:Applied sciences [Multidisciplinary Digital Publishing Institute]
卷期号:10 (14): 4830-4830 被引量:12
标识
DOI:10.3390/app10144830
摘要

In the past few years, research efforts have focused on plant exploitation for deriving some valuable compounds. Extraction has been usually performed using petrochemical and volatile organic solvents, but nowadays, increased recognition of environmental pollution has prompted the utilization of green solvents as alternatives. Therefore, the aim of the present study was to exploit deep eutectic solvents (DES) (choline chloride: citric acid and choline chloride: glucose) as solvents for extracting valuable phenolic antioxidants from grapes. Investigation was conducted on ten grape varieties, observing seeds and skin as different matrix. Total polyphenol content (TPC) was determined by Folin-Ciocalteu spectrophotometric microassay. Antioxidant activity was investigated using four different tests and results were combined in a unique Antioxidant Composite Index (ACI) to reveal comprehensive information about this biological activity. Polyphenol compounds were identified and quantified with the aim of HPLC-diode array detector (DAD). Overall results support that DES (particularly choline chloride: citric acid) were comparable to conventional solvent, and in most cases even outperformed acidified aqueous ethanol (concerning extraction efficiency and antioxidant activity). Regardless of varietal distinctions, grape seeds have higher antioxidant capacity compared to grape skin, and such findings are according to their phenol compound concentrations.
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