骨桥蛋白
电化学
材料科学
化学
医学
内科学
电极
物理化学
作者
Sofia G. Meirinho,Luís G. Dias,António M. Peres,L. R. Rodrigues
出处
期刊:Portugaliae Electrochimica Acta
日期:2018-01-01
卷期号:36 (1): 1-9
被引量:7
标识
DOI:10.4152/pea.201801001
摘要
Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others.This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers.To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN)6] 3-/4-as the redox probe for cyclic voltammetry measurements.The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins.Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.
科研通智能强力驱动
Strongly Powered by AbleSci AI