已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Analysis of key genes and signaling pathways involved in Helicobacter pylori‐associated gastric cancer based on The Cancer Genome Atlas database and RNA sequencing data

幽门螺杆菌 生物 癌症 基因 核糖核酸 小RNA 癌症研究 数据库 遗传学 计算机科学
作者
Yi Hu,Cong He,Jianping Liu,Nianshuang Li,Chao Peng,Yao‐Bin Yang‐Ou,Xiaoyu Yang,Nonghua Lü,Yin Zhu
出处
期刊:Helicobacter [Wiley]
卷期号:23 (5) 被引量:38
标识
DOI:10.1111/hel.12530
摘要

Helicobacter pylori (H. pylori) infection is associated with the development of gastric cancer, although the mechanism is unclear. Herein, this study aimed to clarify the key genes and signaling pathways involved in H. pylori pathogenesis based on The Cancer Genome Atlas (TCGA) database and RNA sequencing analysis.Forty-nine gastric cancer samples (16 with H. pylori and 33 without H. pylori) and 35 cancer-adjacent normal samples from TCGA database were analyzed by bioinformatics. The differentially expressed genes between H. pylori-positive and H. pylori-negative patients were verified in 18 gastric cancer (GC) samples (9 with H. pylori and 9 without H. pylori), which were analyzed using RNA sequencing. Survival analysis was carried out to explore associations between the differentially expressed genes and prognosis. Bioinformatics analysis was performed to determine the signaling pathways associated with H. pylori.The baseline level of clinical features from TCGA database and RNA sequencing showed no differences between the H. pylori-positive and H. pylori-negative GC groups (P > 0.05). TP53 was shown to be upregulated in the H. pylori-positive group in both TCGA database and RNA sequencing data, which also showed higher expression in the GC tissues than in adjacent normal tissues (P < 0.05). CCDC151, CHRNB2, GMPR2, HDGFRP2, and VSTM2L were shown to be downregulated in the H. pylori-positive group by both TCGA database and RNA sequencing, which also showed lower expression in the GC tissues than in adjacent normal tissues (P < 0.05). GC patients with low expression levels of HDGFRP2 had a poor prognosis (P < 0.05). Thirty-three signaling pathways and 10 biological processes were found to be positively associated with H. pylori infection (P < 0.05, FDR < 0.05).These results indicate that some genes (TP53, CCDC151, CHRNB2, GMPR2, HDGFRP2, VSTM2L) and previously unidentified signaling pathways (eg, the Hippo signaling pathway) might play an important role in H. pylori-associated GC.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
寒冷河马完成签到,获得积分10
刚刚
leibaozun完成签到 ,获得积分10
5秒前
6秒前
雷天雨完成签到 ,获得积分10
6秒前
ASASAS完成签到,获得积分10
7秒前
烂漫小蝴蝶完成签到,获得积分10
10秒前
呼延水云发布了新的文献求助10
11秒前
ASASAS发布了新的文献求助50
13秒前
魔幻诗兰完成签到,获得积分10
13秒前
科研通AI2S应助小久小力采纳,获得10
14秒前
小王子完成签到,获得积分10
20秒前
22秒前
qianqian完成签到,获得积分20
28秒前
梅者如西发布了新的文献求助20
28秒前
WXY完成签到,获得积分10
32秒前
小洁完成签到 ,获得积分10
32秒前
小王子发布了新的文献求助10
33秒前
标致一手完成签到 ,获得积分10
34秒前
华仔应助梅者如西采纳,获得10
34秒前
36秒前
梅者如西完成签到,获得积分10
39秒前
超脱闲人发布了新的文献求助10
41秒前
科研通AI2S应助fengyuke采纳,获得10
42秒前
北觅完成签到 ,获得积分10
44秒前
可爱的函函应助七草肃采纳,获得10
46秒前
卧镁铀钳完成签到 ,获得积分10
55秒前
zz完成签到 ,获得积分10
56秒前
青羽凌雪应助fengyuke采纳,获得10
56秒前
舒适怀寒完成签到 ,获得积分10
57秒前
今后应助是你刘大爷采纳,获得10
58秒前
背后的皮带完成签到 ,获得积分10
1分钟前
1分钟前
wang完成签到 ,获得积分10
1分钟前
hh发布了新的文献求助30
1分钟前
1分钟前
良良丸完成签到 ,获得积分10
1分钟前
思源应助彩色的金毛采纳,获得10
1分钟前
wangye完成签到 ,获得积分10
1分钟前
1分钟前
hua完成签到,获得积分10
1分钟前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 890
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3256828
求助须知:如何正确求助?哪些是违规求助? 2898945
关于积分的说明 8303123
捐赠科研通 2568188
什么是DOI,文献DOI怎么找? 1394905
科研通“疑难数据库(出版商)”最低求助积分说明 652924
邀请新用户注册赠送积分活动 630631