Facile room temperature synthesis of ultra-small sized porous organic cages for fluorescent sensing of copper ion in aqueous solution

Facile synthesis of nano porous organic cages with small size and good fluorescence property is highly desirable, but still challenging and scarce for their sensing applications. Here we report a rapid room-temperature recrystallization method for the preparation of nano porous organic cages with ultra-small size as a fluorescent probe for copper ion. The prepared nano porous organic cages gave the diameter of 2.49 ± 0.04 nm, and exhibited stable emission at 535 nm with absolute quantum yield of 0.68%. On the basis of the coordination interaction and charge transfer between the nano porous organic cages and copper ion, a simple fluorescent probe for copper ion in aqueous solution was developed. The developed method gave a calibration function of QE = 0.4815lg[Cu2+] + 0.5847 (where QE is the quenching efficiency; [Cu2+] in μM) (R2 = 0.9987) in a concentration range of 0.1–2 μM, the limit of detection (3s) of 8 nM, and the relative standard deviation of 0.36% for 10 replicate determinations of 0.5 μM copper ion. The recoveries of spiked copper ion in tap water samples ranged from 96.8% to 103.0%. The proposed method possesses good sensitivity, selectivity and accuracy.