已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

DOP79 Biomarkers for IBD using OLINK Proteomics inflammation panel: Preliminary results from the COLLIBRI consortium

生物标志物 炎症性肠病 多路复用 溃疡性结肠炎 蛋白质组学 疾病 医学 内科学 克罗恩病 发病机制 免疫学 计算生物学 生物信息学 生物 遗传学 基因
作者
Tamas Korcsmaros,Benoît L. Salomon,Bram Verstockt,Rocco Ungaro,Konrad Aden,Geert R. D'Haens,K Komori,Heath M. Guay,Mark S. Silverberg,Severine Vermeire,Jonas Halfvarson
出处
期刊:Journal of Crohn's and Colitis [Oxford University Press]
卷期号:16 (Supplement_1): i123-i124 被引量:1
标识
DOI:10.1093/ecco-jcc/jjab232.118
摘要

Abstract Background Circulating serum proteins have provided insights into disease pathogenesis and are being used to identify prognostic, diagnostic and therapeutic biomarkers for chronic inflammatory diseases. With this pilot project, the Collaborative IBD Biomarker Research Initiative (COLLIBRI) consortium aimed to unravel disease heterogeneity in inflammatory bowel disease (IBD). Methods Serum samples were cross-sectionally obtained from 3,390 individuals (Crohn’s disease (CD), n=1815; ulcerative colitis (UC), n=1170; healthy, n=405) recruited at nine centres from Sweden and Belgium. Relative levels of 92 proteins were analysed using the Proseek Multiplex Inflammation I Probe kit 96x96 (Olink Proteomics, Uppsala, Sweden) and reported as arbitrary units, i.e., normalised protein expression on a log2 scale. Using a multivariate integrative approach, we identified protein signatures distinguishing CD and UC samples and attempted to identify clusters or subgroups within patients. Recruiting centre, cohort and batch information were considered for the integrative analysis. Optimisation was performed for identifying the number of components and features per component using 5-fold cross-validation and Leave-One-Group-Out-Cross-Validation, respectively. Information on transcriptional regulators was retrieved from the ReMap project using the orthogonal regulatory resource ChEA3. Results A panel of 8 proteins was identified which could segregate CD and UC patients (Figure 1). FGF19 exhibited a consistent trend of expression (downregulated in CD) across all batches of datasets. An integrated AUC of 72.5% was achieved across the different batches of samples used in the study with the highest AUC (79.2%, P-value 8.5e-07) being recorded for a single batch of samples (CD = 42, UC = 56). On a centre-specific dataset, the cross-centre integrated signature achieved an AUC of 75.1%. We identified three transcription factors (MEF2A, BATF, NFKB2), of which the two latter ones are known to modulate intestinal inflammation and which could potentially regulate the expression of at least half of the genes encoding the proteins in the predictive 8-protein panel. Conclusion We identified an integrated proteomic biomarker panel capable of separating CD and UC patients. Through further integration of confounder variables along with using other supervised and unsupervised approaches, subsequent analyses may further refine the molecular heterogeneity among CD and UC patients. Our results demonstrate the need for large datasets to identify relevant clusters of patients with IBD, since the diagnosis exhibits a high degree of clinical heterogeneity. *Equally contributed
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助wukong采纳,获得10
3秒前
4秒前
7秒前
9秒前
11秒前
12秒前
Rain完成签到,获得积分10
12秒前
李爱国应助科研通管家采纳,获得10
14秒前
小马甲应助科研通管家采纳,获得10
14秒前
深情安青应助科研通管家采纳,获得10
14秒前
天天快乐应助科研通管家采纳,获得10
14秒前
彭于晏应助科研通管家采纳,获得10
14秒前
丘比特应助科研通管家采纳,获得10
14秒前
Rondab应助科研通管家采纳,获得30
14秒前
14秒前
FashionBoy应助科研通管家采纳,获得10
14秒前
16秒前
一直向前发布了新的文献求助10
17秒前
17秒前
可爱的函函应助mellow采纳,获得10
17秒前
20秒前
20秒前
TRY发布了新的文献求助10
21秒前
毕业论文三万字完成签到,获得积分10
22秒前
JOY发布了新的文献求助10
24秒前
momo发布了新的文献求助10
24秒前
晗晗有酒窝完成签到,获得积分10
24秒前
缥缈的碧曼完成签到,获得积分10
25秒前
斯文败类应助Steven采纳,获得10
26秒前
27秒前
28秒前
天天快乐应助大喵采纳,获得10
29秒前
mellow发布了新的文献求助10
31秒前
32秒前
32秒前
非也非也6发布了新的文献求助10
32秒前
JOY完成签到,获得积分10
33秒前
猫瓜西完成签到,获得积分10
33秒前
整齐凝竹完成签到 ,获得积分10
37秒前
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989811
求助须知:如何正确求助?哪些是违规求助? 3531927
关于积分的说明 11255560
捐赠科研通 3270706
什么是DOI,文献DOI怎么找? 1805035
邀请新用户注册赠送积分活动 882181
科研通“疑难数据库(出版商)”最低求助积分说明 809190