离体
类有机物
扁桃体
细胞生物学
生物
体内
免疫学
遗传学
作者
Zachary W. Wagoner,Mahina Tabassum Mitul,Lisa E. Wagar
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 3-13
标识
DOI:10.1007/978-1-0716-3950-4_1
摘要
Tools to study memory B cell (MBC) development and function are needed to understand their role in supporting sustained protection against recurrent infections. While human MBCs are traditionally measured using blood, there is a growing interest in elucidating their behavior within lymphoid tissues, which are the main sites where adaptive immune responses are orchestrated. In this chapter, we introduce a high-throughput organoid system that is derived from primary human lymphoid tissues. The approach can recapitulate many hallmarks of successful adaptive immune responses and capture inter-individual variation in response to a variety of stimuli. Lymphoid tissue organoids enable characterization of pre-existing antigen-specific MBCs within an entirely human system and can provide valuable insights into MBC dynamics.
科研通智能强力驱动
Strongly Powered by AbleSci AI