猪流行性腹泻病毒
病毒学
冠状病毒
生物
自噬
病毒复制
病毒
腹泻
内化
维罗细胞
微生物学
细胞
2019年冠状病毒病(COVID-19)
医学
疾病
遗传学
细胞凋亡
病理
传染病(医学专业)
内科学
作者
Weixiao Zhang,Huiyong Shen,Menglu Wang,Xuelei Fan,Songqi Wang,Nile Wuri,Bin Zhang,Haiyan He,Chunhong Zhang,Zhicheng Liu,Ming Liao,Jianfeng Zhang,Yugu Li,Jianmin Zhang
标识
DOI:10.3389/fmicb.2023.1164851
摘要
Animal and human health are severely threatened by coronaviruses. The enteropathogenic coronavirus, porcine epidemic diarrhea virus (PEDV), is highly contagious, leading to porcine epidemic diarrhea (PED), which causes large economic losses in the world's swine industry. Piglets are not protected from emerging PEDV variants; therefore, new antiviral measures for PED control are urgently required. Herein, the anti-PEDV effects and potential mechanisms of fangchinoline (Fan) were investigated. Fan dose-dependently inhibited a PEDV infection at 24 h post-infection (EC 50 value = 0.67 μM). We found that Fan mainly affected the PEDV replication phase but also inhibited PEDV at the attachment and internalization stages of the viral life cycle. Mechanistically, Fan blocked the autophagic flux in PEDV-infected cells by regulating the expression of autophagy-related proteins and changing PEDV virus particles. In summary, Fan inhibits PEDV infection by blocking the autophagic flux in cells. Our findings will help develop new strategies to prevent and treat PEDV infection.
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