精子
溶血磷脂酰胆碱
顶体反应
顶体
海藻糖
化学
男科
细胞生物学
精液
生物
生物化学
精子
医学
磷脂酰胆碱
磷脂
膜
作者
Ejaz Ahmad,Zahid Naseer,Melih Aksoy,Niyazi Küçük,Uğur Uçan,İlker Serin,Ahmet Ceylan
出处
期刊:Andrologia
[Wiley]
日期:2014-10-01
卷期号:47 (7): 786-792
被引量:14
摘要
This study was aimed to investigate the influence of trehalose on osmotic tolerance and the ability of ram spermatozoon to undergo acrosome reaction induced by lysophosphatidylcholine (LPC). In experiment 1, the diluted ejaculates were exposed to anisosmotic fructose solutions (70, 500, 750 and 1000 mOsm l−1) with or without 50 mm trehalose. The presence of trehalose in hyperosmotic conditions enhanced (P < 0.05) the percentage of live, live-intact and intact spermatozoa. Similarly, trehalose enhanced (P < 0.05) the live and live-intact spermatozoa during hypo-osmotic conditions. In experiment 2, the centrifuged ejaculates were diluted with TCG only or TCG containing either 50 or 100 mm trehalose. The acrosome reaction was induced by LPC. The percentage of acrosome-reacted spermatozoon was less (P < 0.05) in trehalose-supplemented groups compared to control. In experiment 3, the ejaculates were cryopreserved in an extender containing 0 mm (control), 50 mm or 100 mm trehalose. Supplementation of extender with trehalose, either 50 mm or 100 mm, enhanced the cryosurvival rate (P < 0.05) compared to the control. In conclusion, the presence of trehalose in anisosmotic conditions enhances the osmotic tolerance, cryosurvival rate of ram spermatozoon and suppresses their ability to undergo LPC and cryo-induced acrosome reaction.
科研通智能强力驱动
Strongly Powered by AbleSci AI