CYTOKINOGENESIS DISORDERS IN MECHANISMS OF THE EXPERIMENTAL PERODONTITIS DEVELOPMENT AND THEIR CORRECTION BY FLAVONOL

The aim: Evaluation of changes in proinflammatory and anti-inflammatory units of the cytokine profile in the mechanisms of development of experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on its parameters. Materials and methods: Experimental periodontitis was caused by introducing into the tissues of the periodontal complex a mixture of microorganisms diluted with egg protein. In order to enhance the immune response, a complete Freund’s adjuvant was injected into the rat’s paw at the same time. For correction, intramuscular injections of water-soluble quercetin at a dose of 100 mg / kg body weight were performed for 7 days (7th to 14th day). Results: The use of flavonol quercetin led to a decrease in the serum content of experimental animals with pro-inflammatory cytokines. With regard to anti-inflammatory cytokines, their content in the blood of animals during the development of this simulated inflammatory process changed in the opposite direction. Quercetin effectively eliminated the imbalance of the immune system and increased the level of IL-10 and IL-4 in the serum. After injections of quercetin, the relation of pro- and anti-inflammatory cytokines in the serum of animals on 14th day of the study was reduced compared to their content in rats that did not receive correction. Conclusions: The formation and course of experimental bacterial-immune periodontitis is accompanied by a complex of pathological changes in immunocytokinogenesis, which is manifested by a progressive increase in serum concentrations of proinflammatory cytokines and a decrease in anti-inflammatory cytokines. Flavonol quercetin reduces the concentration of pro-inflammatory and increases the content of anti-inflammatory cytokines.