胶质纤维酸性蛋白
琼脂糖
检出限
分子生物学
抗体
材料科学
荧光
线性范围
特异性抗体
色谱法
生物
化学
免疫学
免疫组织化学
物理
量子力学
作者
Yunsu Ma,Guanhong Xu,Fangdi Wei,Yao Cen,Yueyue Song,Yujie Ma,Xiaoman Xu,Menglan Shi,Muhammad Sohail,Qin Hu
出处
期刊:Nanotechnology
[IOP Publishing]
日期:2018-01-31
卷期号:29 (14): 145501-145501
被引量:31
标识
DOI:10.1088/1361-6528/aaabea
摘要
Glial fibrillary acidic protein (GFAP) is expressed in the central nervous system and the level of GFAP normally rises with brain injury and astroglial tumors. So, serum GFAP is used as a marker for diagnosing various types of brain damage and astroglial tumors. In this study, a new sensor based on carbon dots (CDs) linked with antibodies to specifically detect GFAP in human serum was developed. Anti-GFAP (Ab1) linked with protein A/G agarose resin (PA/G) as a capture antibody (PA/G-Ab1) and anti-GFAP (Ab2) labeled with CDs as a detection antibody (CDs-Ab2) were prepared firstly. Then the CD-linked antibody immunosorbent assay (CLAISA) method was constructed based on the sandwich conjunction reaction among PA/G-Ab1, GFAP, and CDs-Ab2. CLAISA, using the fluorescence of PA/G-Ab1-GFAP-Ab2-CDs as the direct signal, enabled the proposed immunosensor to detect GFAP sensitively with a linear range of 0.10-8.00 ng ml-1 and a detection limit of 25 pg ml-1. This method was applied to the determination of GFAP in human serum by the standard addition method, and the results showed high accuracy and precision. Considering the easy synthetic process and excellent performance of CLAISA, this method has great potential to be used to monitor GFAP in the clinic.
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