艾美球虫
生物
聚合酶链反应
底漆(化妆品)
粪便
套式聚合酶链反应
基因组DNA
病菌
病毒学
复式(建筑)
球虫
基因
DNA
微生物学
遗传学
寄生虫寄主
化学
万维网
有机化学
计算机科学
作者
Fitrine Ekawasti,M F Nashrulloh,Wisnu Nurcahyo,Dwi Priyowidodo,Joko Prastowo,Lintang Winantya Firdausy
出处
期刊:IOP conference series
[IOP Publishing]
日期:2022-02-01
卷期号:976 (1): 012008-012008
被引量:2
标识
DOI:10.1088/1755-1315/976/1/012008
摘要
Abstract Eimeria bovis and Eimeria zuernii are highly pathogenic Eimeria species in cattle that are the most prevalent causes of a severe clinical illness characterized by hemorrhagic diarrhea in calves and young cattle with potentially fatal effects over the world. The oocysts of a handful of the known bovine eimeriosis species are difficult to distinguish morphologically. For the specific differentiating evidence of Eimeria species, symptomatic research institutions are increasingly relying on DNA-based technologies. This research offers a duplex polymerase chain reaction (PCR) test based on the internal transcribed spacer-1 (ITS-1) gene that may be used to diagnose E. bovis and E. zuernii in cattle from various locations at the same time. The oocysts were concentrated and purified using a fecal harvesting method. The genomic DNA is extracted according to the instructions included with the kit. Primer pairs specific to each species, as well as a standard optimum annealing temperature of 55°C for these species, were discovered. The samples were amplified in a homogenous way, resulting in a homogeneous band ladder, revealing that the test could distinguish between two highly pathogenic Eimeria species in one tube reaction. This duplex PCR assay can detect a high pathogenic bovine Eimeria simultaneously in a rapid and low cost.
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