Response of DBL-1/TGF-β signaling-mediated neuron-intestine communication to nanopolystyrene in nematode Caenorhabditis elegans

TGF-β signaling pathway is important for the regulation of stress response in organisms. We here used Caenorhabditis elegans to determine the function of DBL-1/TGF-β signaling pathway in the control of response to nanopolystyrene (100 nm). In DBL-1/TGF-β signaling pathway, exposure to 1-1000 μg/L nanopolystyrene significantly increased the expressions of dbl-1 encoding a TGF-β ligand, sma-6 encoding a TGF-β receptor, sma-4 encoding a Co-Smad, and two genes (mab-31 and sma-9) encoding transcriptional factors. DBL-1 acted in the neurons to control the response to nanopolystyrene. In the neurons, the expression and the function of DBL-1 were under the control of two signaling cascades (SMOC-1-ZAG-1 and SMOC-1-ADT-2). TGF-β receptor SMA-6 acted in the intestine to control the response to nanopolystyrene. The downstream Co-Smad/SMA-4 and two transcriptional factors (MAB-31 and SMA-9) of SMA-6 in the intestine were further identified to be required for the control of response to nanopolystyrene. In nanopolystyrene exposed nematodes, intestinal MAB-31 activated the mitochondrial Mn-SOD/SOD-3 by modulating DAF-16 activity, and intestinal SMA-9 activated the mitochondrial unfolded protein response by affecting ELT-2 activity. Therefore, the DBL-1/TGF-β signaling pathway mediated an important neuron-intestine communication in nanopolystyrene exposed nematodes.