凝聚
表面改性
癌细胞
细胞
抗体依赖性细胞介导的细胞毒性
化学
单克隆抗体
抗体
细胞外基质
材料科学
癌症
免疫学
生物化学
生物
物理化学
遗传学
作者
Hongfei Chen,Yishu Bao,Jing Wang,Fang-Zhi Chen,Ryohichi Sugimura,Xiangze Zeng,Jiang Xia
标识
DOI:10.1002/ange.202410566
摘要
Cell therapies such as CAR‐T have demonstrated significant clinical successes, driving the investigation of immune cell surface engineering using natural and synthetic materials to enhance their therapeutic performance. However, many of these materials do not fully replicate the dynamic nature of the extracellular matrix (ECM). This study presents a cell surface engineering strategy that utilizes phase‐separated peptide coacervates to decorate the surface of immune cells. We meticulously designed a tripeptide, Fmoc‐Lys‐Gly‐Dopa‐OH (KGdelta; Fmoc = fluorenylmethyloxycarbonyl; delta = Dopa, dihydroxyphenylalanine), that forms coacervates in aqueous solution via phase separation. These coacervates, mirroring the phase separation properties of ECM proteins, coat the natural killer (NK) cell surface with the assistance of Fe3+ ions and create an outer layer capable of encapsulating monoclonal antibodies (mAb), such as Trastuzumab. The antibody‐embedded coacervate layer equips the NK cells with the ability to recognize cancer cells and eliminate them through enhanced antibody‐dependent cellular cytotoxicity (ADCC). This work thus presents a unique strategy of cell surface functionalization and demonstrates its use in displaying cancer‐targeting mAb for cancer therapies, highlighting its potential application in the field of cancer therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI