Xueshuantong injection alleviates cerebral microcirculation disorder in middle cerebral artery occlusion/reperfusion rats by suppressing inflammation via JNK mediated JAK2/STAT3 and NF-κB signaling pathways

三七 微循环 医学 血脑屏障 炎症 药理学 病理 内科学 中枢神经系统 替代医学
作者
Gaorui Wang,Ziyu Chen,Yingying Song,Hui Wu,Ming Chen,Shusheng Lai,Xiaojun Wu
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:298: 115592-115592 被引量:19
标识
DOI:10.1016/j.jep.2022.115592
摘要

In the long history of traditional Chinese medicine, Panax notoginseng has been used as a key herb for the treatment of blood diseases. Brain microvessels support adequate blood circulation to maintain normal physiological function, therefore, brain microcirculation disorder is an important therapeutic target for various brain diseases. However, the role of Xueshuantong (XST) injection composed of saponins from P. Notoginseng (PNS) in the amelioration of cerebral microcirculation disorder is unclear.Cerebral microcirculation disorder and inflammation play a vital role in stroke. Capillary endothelial cells and adjacent tight junctions are fundamental to the structure and function of cerebrovascule. XST injection has been used clinically in the treatment of stroke, but no studies have reported its indication in cerebral microcirculation disorder. This study is to explore the action and mechanism of XST injection in the alleviation of cerebral microcirculation disorder in middle cerebral artery occlusion/reperfusion (MCAO/R) rats.MCAO/R rats and LPS-induced bEnd.3 cells were employed for the investigation of effect and mechanism of XST injection. Brain damages were evaluated by neurobehavioral assessment, 2, 3, 5-triphenyltetrazolium chloride (TTC) staining, hematoxylin and eosin staining (H&E), and Nissl staining. Morphology and density changes of cerebral microvessels were monitored by immunohistochemistry. Cell permeability was detected by measurement of trans-endothelial electrical resistance (TEER) and sodium fluorescein (NaF) leakage. The mRNA and protein expressions of inflammatory cytokines, tight junction proteins, adhesion molecules, Janus kinase 2 (JAK2), signal transducer and activator of transcription-3 (STAT3), inhibitor of NF-κB (IκB), nuclear factor-κB (NF-κB) and c-jun N-terminal kinase (JNK) in brain microvessels and lipopolysaccharide (LPS)-induced bEnd.3 cells were measured by real-time PCR and Western blot, respectively.XST injection at 48 mg/kg significantly improved the neurological damage, inflammatory infiltration, and microvessel morphology, and increased microvessel density in brain of MCAO/R rats. The endothelial permeability was significantly mitigated by XST injection in LPS-induced bEnd.3 cells. Meanwhile, the tight junction proteins such as zona occludens 1 (ZO-1) and occludin were elevated remarkably in brain microvessel of MCAO/R rats and LPS-induced bEnd.3 cells. Moreover, the expression of inflammatory mediators including interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), cycloocygenases 2 (COX-2), vascular cellular adhesion molecule-1 (VCAM-1), matrix metalloproteinase (MMP)-2, and MMP-9 were inhibited by XST injection. In addition, XST injection suppressed the phosphorylation of JAK2, STAT3, IκB, NF-κB and JNK, which could be abolished by anisomycin, the JNK agonist.XST injection improved cerebral microvescular structure damage and dysfunction in MCAO/R rats through inhibiting inflammation activated by JNK mediated JAK2/STAT3 and NF-κB signaling pathways. The novel findings may provide theoretical basis for the clinical application in the treatment of cerebral microcirculation disorder.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
北风完成签到,获得积分10
1秒前
1秒前
suwan完成签到,获得积分10
1秒前
DCOI完成签到,获得积分10
1秒前
3秒前
3秒前
本草石之寒温完成签到 ,获得积分10
3秒前
晚云烟月完成签到,获得积分10
3秒前
山羊穿毛衣完成签到,获得积分0
3秒前
4秒前
Orange应助秋秋糖xte采纳,获得10
4秒前
苏槑特完成签到,获得积分10
4秒前
maomao1986完成签到,获得积分10
4秒前
ambrose37完成签到 ,获得积分10
4秒前
完美的友蕊应助达到采纳,获得10
5秒前
5秒前
shanshan发布了新的文献求助30
6秒前
Q_Q发布了新的文献求助50
6秒前
zzzkyt发布了新的文献求助10
7秒前
disciple完成签到,获得积分10
7秒前
踏月偷心发布了新的文献求助10
7秒前
hkunyu完成签到 ,获得积分10
7秒前
雨无意完成签到,获得积分10
8秒前
ymxlcfc完成签到 ,获得积分10
8秒前
犹豫小海豚完成签到,获得积分10
8秒前
墨之默完成签到,获得积分10
8秒前
9秒前
9秒前
热塑性复合材料完成签到 ,获得积分20
10秒前
酷小裤完成签到,获得积分10
10秒前
黄淮科研小白龙完成签到 ,获得积分10
11秒前
12秒前
爆米花应助Xuancheng_SINH采纳,获得10
12秒前
wdn0411完成签到,获得积分10
12秒前
宝玉完成签到 ,获得积分10
13秒前
Once完成签到,获得积分10
13秒前
Lml完成签到,获得积分10
14秒前
14秒前
li完成签到 ,获得积分10
14秒前
14秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968593
求助须知:如何正确求助?哪些是违规求助? 3513416
关于积分的说明 11167791
捐赠科研通 3248853
什么是DOI,文献DOI怎么找? 1794507
邀请新用户注册赠送积分活动 875170
科研通“疑难数据库(出版商)”最低求助积分说明 804671