自噬
骨关节炎
软骨细胞
自愈水凝胶
壳聚糖
软骨
材料科学
离体
化学
生物医学工程
体内
癌症研究
医学
病理
生物
生物化学
解剖
细胞凋亡
高分子化学
生物技术
替代医学
作者
Pengfei Chen,Chen Xia,Sheng Mei,Jiying Wang,Zhi‐Xin Shan,Xianfeng Lin,Shunwu Fan
出处
期刊:Biomaterials
[Elsevier]
日期:2016-03-01
卷期号:81: 1-13
被引量:112
标识
DOI:10.1016/j.biomaterials.2015.12.006
摘要
Reduced expression of autophagy regulators has been observed in pathological cartilage in humans and mice. The present study aimed to investigate the synergistic therapeutic effect of promotion of chondrocyte autophagy via exposure to sinomenium (SIN) encapsulated by chitosan microspheres (CM-SIN) and photo-crosslinked gelatin methacrylate (GelMA) hydrogel, with the goal of evaluating CM-SIN as a treatment for patients with osteoarthritis. First, we fabricated and characterized GelMA hydrogels and chitosan microspheres. Next, we measured the effect of SIN on cartilage matrix degradation induced by IL1-β in chondrocytes and an ex vivo model. SIN ameliorated the pathological changes induced by IL1-β at least partially through activation of autophagy. Moreover, we surgically induced osteoarthritis in mice, which were injected intra-articularly with CM-SIN and GelMA. Cartilage matrix degradation and chondrocyte autophagy were evaluated 4 and 8 weeks after surgery. Treatment with the combination of CM-SIN and GelMA retarded the progression of surgically induced OA. SIN ameliorated cartilage matrix degradation at least partially by inducing autophagy in vivo. Our results demonstrate that injection of the combination of GelMA hydrogel and CM-SIN could be a promising strategy for treating patients with osteoarthritis.
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