枯草芽孢杆菌
地衣芽孢杆菌
发酵
固态发酵
食品科学
豆粕
蛋白酶
细菌
拉伤
淀粉酶
芽孢杆菌(形态)
嗜热菌
芽孢杆菌目
人口
生物
化学
微生物学
生物化学
酶
原材料
人口学
社会学
解剖
遗传学
生态学
作者
Dandan Liu,Yiting Guo,Yolandani,Haile Ma,Muthupandian Ashokkumar
标识
DOI:10.1016/j.fbio.2023.103154
摘要
This study aimed to investigate the dynamic changes in bacterial communities during natural solid-state fermentation (NSSF) of soybean meal (SBM) and isolate dominant bacteria for peptide production. Results revealed that during NSSF, bacterial diversity gradually decreased, with Bacillus (particularly Bacillus subtilis) dominating the microbial population (over 90%). Three dominant strains were isolated from the fermented SBM and identified as Bacillus subtilis SBM_1, Bacillus licheniformis, and Bacillus subtilis SBM_2, respectively. Bacillus subtilis (Strains 1 and 3) could secrete extracellular protease, amylase, and cellulase at a relatively high temperature (45 °C). In contrast, Bacillus licheniformis (strain 2) could withstand temperatures up to 55 °C but showed limited protease and amylase activities. Furthermore, among the three dominant strains, strain 1 exhibited the highest protein conversion rate of 34.57% after incubating in casein medium for 24 h, followed by strain 3 (18.97%). The peptide yield of the fermented SBM after inoculating three dominant strains significantly (p < 0.05) increased by 23.47%, 7.27%, and 12.20%, respectively, compared with naturally fermented SBM. As a thermophilic peptide-producing strain, strain 1 (named Bacillus subtilis SBM_1) possesses the potential for improving the added value of the SBM in the SSF industry.
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