光刺激
光遗传学
神经科学
双光子激发显微术
物理
钙显像
视皮层
视区
计算机科学
光学
化学
生物
计算机视觉
荧光
有机化学
钙
作者
Nicolò Accanto,Francois G C Blot,Antonio Lorca-Cámara,Valeria Zampini,Florence Phuong Bui,Christophe Tourain,Noam Badt,Ori Katz,Valentina Emiliani
出处
期刊:Neuron
[Elsevier]
日期:2022-11-01
卷期号:111 (2): 176-189.e6
被引量:2
标识
DOI:10.1016/j.neuron.2022.10.030
摘要
•2P-FENDO achieves photostimulation at single-cell resolution in freely moving mice •2P-FENDO demonstrates reliable calcium imaging at 20 Hz in freely moving mice •Intrinsic temporal properties of fiber bundles enable single-cell axial resolution •2P-FENDO reaches 2P imaging until a depth of 150 μm in the mouse brain We developed a flexible two-photon microendoscope (2P-FENDO) capable of all-optical brain investigation at near cellular resolution in freely moving mice. The system performs fast two-photon (2P) functional imaging and 2P holographic photostimulation of single and multiple cells using axially confined extended spots. Proof-of-principle experiments were performed in freely moving mice co-expressing jGCaMP7s and the opsin ChRmine in the visual or barrel cortex. On a field of view of 250 μm in diameter, we demonstrated functional imaging at a frame rate of up to 50 Hz and precise photostimulation of selected groups of cells. With the capability to simultaneously image and control defined neuronal networks in freely moving animals, 2P-FENDO will enable a precise investigation of neuronal functions in the brain during naturalistic behaviors. We developed a flexible two-photon microendoscope (2P-FENDO) capable of all-optical brain investigation at near cellular resolution in freely moving mice. The system performs fast two-photon (2P) functional imaging and 2P holographic photostimulation of single and multiple cells using axially confined extended spots. Proof-of-principle experiments were performed in freely moving mice co-expressing jGCaMP7s and the opsin ChRmine in the visual or barrel cortex. On a field of view of 250 μm in diameter, we demonstrated functional imaging at a frame rate of up to 50 Hz and precise photostimulation of selected groups of cells. With the capability to simultaneously image and control defined neuronal networks in freely moving animals, 2P-FENDO will enable a precise investigation of neuronal functions in the brain during naturalistic behaviors.
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