Reproductive endocrine variation in female olive flounder with different stress coping styles during the breeding season

Aquaculture ResearchVolume 53, Issue 11 p. 3949-3961 ORIGINAL ARTICLE Reproductive endocrine variation in female olive flounder with different stress coping styles during the breeding season Pengxin Jiang, Pengxin Jiang National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorJinjie Guo, Jinjie Guo National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorAqin Chen, Aqin Chen National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorWeiqun Lu, Corresponding Author Weiqun Lu wqlv@shou.edu.cn orcid.org/0000-0002-1949-3496 National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, China Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China Correspondence Weiqun Lu, National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), 201306 Shanghai, China. Email: wqlv@shou.edu.cnSearch for more papers by this author Pengxin Jiang, Pengxin Jiang National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorJinjie Guo, Jinjie Guo National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorAqin Chen, Aqin Chen National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, ChinaSearch for more papers by this authorWeiqun Lu, Corresponding Author Weiqun Lu wqlv@shou.edu.cn orcid.org/0000-0002-1949-3496 National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, China Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China Correspondence Weiqun Lu, National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), 201306 Shanghai, China. Email: wqlv@shou.edu.cnSearch for more papers by this author First published: 23 May 2022 https://doi.org/10.1111/are.15898 Pengxin Jiang and Jinjie Guo joint first authors. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The hypothalamic–pituitary–inter-renal (HPI) axis activity is related to the stress coping styles of olive flounder, Paralichthys olivaceus. But the relationship between hypothalamic–pituitary–gonadal (HPG) axis activity and stress coping styles remains unknown. Here, we combine physiological measurements with ovarian development to examine the reproduction differences between proactive and reactive flounders. The GSI was significantly higher in the proactive fish than the reactive type during the breeding season. In April, all the proactive fish under investigation had ovaries at mid-vitellogenic stage while reactive fish had ovaries at pre-vitellogenic stage, but both the proactive fish and the reactive ones had ovaries at post-vitellogenic stage in May. In HPG axis, the plasma E2 levels and the mRNA levels of fshβ, fshr and lhcgr was higher in the proactive flounder than the reactive ones in April yet there was no difference between them in May. In HPI axis, the concentration of cortisol was lower in the proactive fish than the reactive ones in April, yet the results indicate no difference in May. Surprisingly, the transcript gr in ovary displays no differences between the proactive and the reactive fish and mr shows significant up-regulation for the proactive fish in April and the results indicated no difference again in May. Overall, these data suggested that the proactive individuals matured earlier than the reactive ones, the activity of HPI axis was lower, and the activity of HPG axis was higher in the proactive fish than the reactive ones during the breeding season. Open Research DATA AVAILABILITY STATEMENT Upon reasonable demand, the data supporting the results of this study are obtainable from the corresponding author. Volume53, Issue11August 2022Pages 3949-3961 RelatedInformation