鲍曼不动杆菌
生物膜
微生物学
噬菌体疗法
效价
抗菌剂
抗生素
抗药性
细菌
不动杆菌
孵化
化学
生物
噬菌体
病毒学
铜绿假单胞菌
大肠杆菌
病毒
基因
生物化学
遗传学
作者
Natalia Bagińska,Ilona Grygiel,Filip Orwat,Marek Harhala,Adam Jędrusiak,Elżbieta Gębarowska,Sławomir Letkiewicz,Andrzej Górski,Ewa Jończyk‐Matysiak
标识
DOI:10.1038/s41598-024-54469-z
摘要
Acinetobacter baumannii is currently a serious threat to human health, especially to people with immunodeficiency as well as patients with prolonged hospital stays and those undergoing invasive medical procedures. The ever-increasing percentage of strains characterized by multidrug resistance to widely used antibiotics and their ability to form biofilms make it difficult to fight infections with traditional antibiotic therapy. In view of the above, phage therapy seems to be extremely attractive. Therefore, phages with good storage stability are recommended for therapeutic purposes. In this work, we present the results of studies on the stability of 12 phages specific for A. baumannii under different conditions (including temperature, different pH values, commercially available disinfectants, essential oils, and surfactants) and in the urine of patients with urinary tract infections (UTIs). Based on our long-term stability studies, the most optimal storage method for the A. baumannii phage turned out to be - 70 °C. In contrast, 60 °C caused a significant decrease in phage activity after 1 h of incubation. The tested phages were the most stable at a pH from 7.0 to 9.0, with the most inactivating pH being strongly acidic. Interestingly, ethanol-based disinfectants caused a significant decrease in phage titers even after 30 s of incubation. Moreover, copper and silver nanoparticle solutions also caused a decrease in phage titers (which was statistically significant, except for the Acba_3 phage incubated in silver solution), but to a much lesser extent than disinfectants. However, bacteriophages incubated for 24 h in essential oils (cinnamon and eucalyptus) can be considered stable.
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