群体感应
化学
生物膜
毒力
铜绿假单胞菌
微生物学
分泌物
人口
细菌
病菌
共价键
毒力因子
激活剂(遗传学)
基因
生物化学
遗传学
生物
人口学
有机化学
社会学
作者
Neri Amara,Roi Mashiach,Dotan Amar,Pnina Krief,Stéphane A. Spieser,Matthew J. Bottomley,Amir Aharoni,Michaël M. Meijler
摘要
Chemical coordination of gene expression among bacteria as a function of population density is regulated by a mechanism known as 'quorum sensing' (QS). QS in Pseudomonas aeruginosa, an opportunistic pathogen that causes disease in immunocompromised patients, is mediated by binding of the transcriptional activator, LasR, to its ligand, 3-oxo-C12-HSL, leading to population-wide secretion of virulence factors and biofilm formation. We have targeted QS in P. aeruginosa with a set of electrophilic probes designed to covalently bind Cys79 in the LasR binding pocket, leading to specific inhibition of QS-regulated gene expression and concomitant reduction of virulence factor secretion and biofilm formation. This first example of covalent modification of a QS receptor provides a new tool to study molecular mechanisms of bacterial group behavior and could lead to new strategies for targeting bacterial virulence.
科研通智能强力驱动
Strongly Powered by AbleSci AI