卵清蛋白
PLGA公司
佐剂
免疫系统
脾细胞
免疫增强剂
体内
流式细胞术
抗原
乙醇酸
化学
体外
药理学
乳酸
免疫学
生物
生物化学
生物技术
遗传学
细菌
作者
Li Luo,Tao Qin,Yifan Huang,Sisi Zheng,Ruonan Bo,Zhenguang Liu,Jie Xing,Yuanliang Hu,Jiaguo Liu,Deyun Wang
出处
期刊:Drug Delivery
[Informa]
日期:2017-01-01
卷期号:24 (1): 1099-1111
被引量:35
标识
DOI:10.1080/10717544.2017.1359861
摘要
Biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) has been approved by the US Food and Drug Administration and has frequently been used to develop potential vaccine delivery systems. The immunoregulation and immunopotentiation of Chinese yam polysaccharide (CYP) have been widely demonstrated. In the current study, cell uptake mechanisms in dendritic cells (DCs) were monitored in vitro using confocal laser scanning microscopy, transmission electron microscopy, and flow cytometry. To study a CYP-PLGA nanoparticle-adjuvanted delivery system, CYP and ovalbumin (OVA) were encapsulated in PLGA nanoparticles (CYPPs) to act as a vaccine, and the formulation was tested in immunized mice. The CYPPs more easily underwent uptake by DCs in vitro, and CYPP/OVA could stimulate more effective antigen-specific immune responses than any of the single-component formulations in vivo. Mice immunized using CYPP/OVA exhibited more secretion of OVA-specific IgG antibodies, better proliferation, and higher cytokine secretion by splenocytes and significant activation of CD3+CD4+ and CD3+CD8+ T cells. Overall, the CYPP/OVA formulation produced a stronger humoral and cellular immune response and a mixed Th1/Th2 immune response with a greater Th1 bias in comparison with the other formulations. In conclusion, the data demonstrate that the CYPP-adjuvanted delivery system has the potential to strengthen immune responses and lay the foundation for novel adjuvant design.
科研通智能强力驱动
Strongly Powered by AbleSci AI