Application of engineered myoglobins for biosynthesis of clofazimine by integration with chemical synthesis

Engineered myoglobins (Mbs), such as F43Y/T67R Mb, were applied to catalyze the oxidative coupling of N -4-CPBDA to produce N -5-CCPIPA in the presence of H 2 O 2 , which was further combined with chemical synthesis for the total synthesis of CFZ.