自愈水凝胶
软骨发生
明胶
化学
软骨细胞
组织工程
生物医学工程
活力测定
细胞外基质
肝素
高分子化学
生物物理学
细胞
生物化学
体外
医学
生物
作者
Gabriella C. J. Brown,Khoon S. Lim,Brooke L. Farrugia,Gary J. Hooper,Tim B. F. Woodfield
标识
DOI:10.1002/mabi.201700158
摘要
Abstract Multicomponent gelatin‐methacryloyl (GelMA) hydrogels are regularly adopted for cartilage tissue engineering (TE) applications, where optimizing chemical modifications for preserving biofunctionality is often overlooked. This study investigates the biological effect of two different modification methods, methacrylation and thiolation, to copolymerize GelMA and heparin. The native bioactivity of methacrylated heparin (HepMA) and thiolated heparin (HepSH) is evaluated via thromboplastin time and heparan sulfate‐deficient myeloid cell‐line proliferation assay, demonstrating that thiolation is superior for preserving anticoagulation and growth factor signaling capacity. Furthermore, incorporating either HepMA or HepSH in chondrocyte‐laden GelMA hydrogels, cultured for 5 weeks under chondrogenic conditions, promotes cell viability and chondrocyte phenotype. However, only GelMA‐HepSH hydrogels yield significantly greater differentiation and matrix deposition in vitro compared to GelMA. This study demonstrates that thiol‐ene chemistry offers a favorable strategy for incorporating bioactives into gelatin hydrogels as compared to methacrylation while furthermore highlighting GelMA‐HepSH hydrogels as candidates for cartilage TE applications.
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