Dietary vitamin C requirement of juvenile grass carp ( Ctenopharyngodon idella ) and its effects on growth attributes, organ indices, whole‐body composition and biochemical parameters

Aquaculture NutritionVolume 27, Issue 6 p. 1903-1911 ORIGINAL ARTICLE Dietary vitamin C requirement of juvenile grass carp (Ctenopharyngodon idella) and its effects on growth attributes, organ indices, whole-body composition and biochemical parameters Muhammad Fazail Nasar, Muhammad Fazail Nasar Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this authorSyed Zakir Hussain Shah, Corresponding Author Syed Zakir Hussain Shah dr.zakirhussain@uog.edu.pk Department of Zoology, University of Gujrat, Gujrat, Pakistan Correspondence Syed Zakir Hussain Shah, Department of Zoology, University of Gujrat, Gujrat, Pakistan. Email: dr.zakirhussain@uog.edu.pk and Muhammad Bilal, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China. bilaluaf@hyit.edu.cnSearch for more papers by this authorKiran Aftab, Kiran Aftab Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this authorMahroze Fatima, Mahroze Fatima Department of Fisheries & Aquaculture, University of Veterinary & Animal Sciences, Lahore, PakistanSearch for more papers by this authorMuhammad Bilal, Corresponding Author Muhammad Bilal bilaluaf@hyit.edu.cn orcid.org/0000-0001-5388-3183 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China Correspondence Syed Zakir Hussain Shah, Department of Zoology, University of Gujrat, Gujrat, Pakistan. Email: dr.zakirhussain@uog.edu.pk and Muhammad Bilal, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China. bilaluaf@hyit.edu.cnSearch for more papers by this authorMubashar Hussain, Mubashar Hussain Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this author Muhammad Fazail Nasar, Muhammad Fazail Nasar Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this authorSyed Zakir Hussain Shah, Corresponding Author Syed Zakir Hussain Shah dr.zakirhussain@uog.edu.pk Department of Zoology, University of Gujrat, Gujrat, Pakistan Correspondence Syed Zakir Hussain Shah, Department of Zoology, University of Gujrat, Gujrat, Pakistan. Email: dr.zakirhussain@uog.edu.pk and Muhammad Bilal, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China. bilaluaf@hyit.edu.cnSearch for more papers by this authorKiran Aftab, Kiran Aftab Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this authorMahroze Fatima, Mahroze Fatima Department of Fisheries & Aquaculture, University of Veterinary & Animal Sciences, Lahore, PakistanSearch for more papers by this authorMuhammad Bilal, Corresponding Author Muhammad Bilal bilaluaf@hyit.edu.cn orcid.org/0000-0001-5388-3183 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China Correspondence Syed Zakir Hussain Shah, Department of Zoology, University of Gujrat, Gujrat, Pakistan. Email: dr.zakirhussain@uog.edu.pk and Muhammad Bilal, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China. bilaluaf@hyit.edu.cnSearch for more papers by this authorMubashar Hussain, Mubashar Hussain Department of Zoology, University of Gujrat, Gujrat, PakistanSearch for more papers by this author First published: 26 August 2021 https://doi.org/10.1111/anu.13327Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Vitamin C or ascorbic acid (ASA) is an essential micronutrient for normal growth, antioxidant capacity and immunity of fish. However, appropriate requirements of ASA for Ctenopharyngodon idella, grass carp juveniles (8.46 ± 0.19), remain unknown. For this purpose, a feeding trial of 60 days was conducted in triplicates. Six isolipidic and isoproteic diets containing 0, 25, 50, 100, 200 and 400 mg/kg of ASA were prepared. Results showed a linear increase in growth as ASA supplementation was increased from 0 to 100 mg/kg. However, a further increase in dietary ASA supplementation from 200 to 400 mg/kg resulted in a slight decrease in growth parameters. A similar trend was also recorded for the whole body, proximate composition, antioxidant enzymes and organ indices against ASA supplementation. On the contrary, fish fed with basal diet had higher thiobarbituric acid reactive substances (TBARS) content in the liver and muscles than those fed with ASA supplemented diets. ASA concentration in muscles increased in a dose-dependent manner, while ASA value was the highest in the fish fed with 100 mg/kg ASA concentration. The weight gain (WG%) data were subjected to broken-line regression analysis, and the optimal ASA requirement of grass carp juveniles was estimated to be 67.1667 mg/kg. Taken together, the inclusion of 67.1667 mg/kg of ASA is recommended in the feed of grass carp juveniles for optimum growth performance. We also recommend analysing the effect of ASA in combination with vitamin A and E. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Volume27, Issue6December 2021Pages 1903-1911 RelatedInformation