染色
细胞外基质
免疫荧光
类有机物
抗体
生物
病理
化学
细胞生物学
免疫学
医学
作者
Hüseyin Erdinç Beşikcioğlu,Ümmügülsüm Yurteri,Linhan Ye,Fangfang Zhang,Alessandra Moretti,Ibrahim Halil Gürçınar,Alper Doğruöz,Didem Karakaş,Helmut Frieß,Güralp O. Ceyhan,Rouzanna Istvánffy,İhsan Ekin Demir
出处
期刊:STAR protocols
[Elsevier]
日期:2024-06-01
卷期号:5 (2): 103132-103132
标识
DOI:10.1016/j.xpro.2024.103132
摘要
The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibodies and mounting.
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