体内
癌症研究
白细胞介素12
淋巴因子激活杀伤细胞
体外
归巢(生物学)
细胞毒性T细胞
白细胞介素21
细胞疗法
免疫学
生物
干细胞
细胞生物学
生态学
生物化学
生物技术
作者
Farzaneh Sharifzad,Soura Mardpour,Saeid Mardpour,Esmaeil Fakharian,Adeleh Taghikhani,Amirhossein Sharifzad,Sahar Kiani,Yasaman Heydari,Marek Łos,Zahra Azizi,Saeid Ghavami,Amir Ali Hamidieh,Marzieh Ebrahimi
摘要
Natural killer (NK) cell therapy is one of the most promising treatments for Glioblastoma Multiforme (GBM). However, this emerging technology is limited by the availability of sufficient numbers of fully functional cells. Here, we investigated the efficacy of NK cells that were expanded and treated by interleukin-2 (IL-2) and heat shock protein 70 (HSP70), both in vitro and in vivo. Proliferation and cytotoxicity assays were used to assess the functionality of NK cells in vitro, after which treated and naïve NK cells were administrated intracranially and systemically to compare the potential antitumor activities in our in vivo rat GBM models. In vitro assays provided strong evidence of NK cell efficacy against C6 tumor cells. In vivo tracking of NK cells showed efficient homing around and within the tumor site. Furthermore, significant amelioration of the tumor in rats treated with HSP70/Il-2-treated NK cells as compared to those subjected to nontreated NK cells, as confirmed by MRI, proved the efficacy of adoptive NK cell therapy. Moreover, results obtained with systemic injection confirmed migration of activated NK cells over the blood brain barrier and subsequent targeting of GBM tumor cells. Our data suggest that administration of HSP70/Il-2-treated NK cells may be a promising therapeutic approach to be considered in the treatment of GBM.
科研通智能强力驱动
Strongly Powered by AbleSci AI