细胞外小泡
聚集诱导发射
小泡
纳米技术
荧光
跟踪(教育)
细胞外
化学
生物物理学
材料科学
膜
生物
细胞生物学
生物化学
物理
光学
教育学
心理学
作者
Zihao Ou,Xiaojing He,Qianbei Li,Nannan Cao,Meng Gao,Bairong He,Minying Zhang,Fang Hu,Weirong Yao,Qian Wang,Lei Zheng,Bo Situ
标识
DOI:10.1016/j.cej.2022.136847
摘要
• High-performance tracking of BEVs in living systems has been realized with an AIEgen. • The probe can illuminate BEVs and shows remarkable merits over traditional methods. • The spatial and temporal distributions of BEVs within host can be clearly visualized. The nano-sized, membrane-enclosed bacterial extracellular vesicles (BEVs) released by bacteria are now regarded as important mediators involved in various physiological and pathological processes of human. To elucidate their roles in bacteria-to-host communication, methods for visualizing and tracking of these nano-vesicles are crucial, but ideal means are lacking currently. In this work, we report the development of a bioprobe, DPASP, for BEV bioimaging with superior performance. DPASP can target BEVs with boosted fluorescence due to its aggregation-induced emission (AIE) attribute, which allows BEV labeling with a high signal-to-background ratio. For BEV imaging, DPASP outperforms the commercial dyes in terms of labeling efficiency, retention time, photostability, and biocompatibility. The applications of DPASP in studying the spatial and temporal distributions of E. coli -derived BEVs within living cells and mice were also demonstrated. The approach developed here provides a valuable tool to better understand the roles of BEVs in health and diseases.
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