纳米载体
内化
卵巢癌
DNA
纳米技术
细胞生物学
癌细胞
核糖核酸
癌症
DNA折纸
小RNA
化学
癌症研究
生物
生物物理学
细胞
药物输送
材料科学
遗传学
基因
生物化学
作者
Li-ting Han,Tao Song,Xinyu Wang,Yan Luo,Chuanqi Gu,Xin Tao Li,Jin-da Wen,Wen Zhi-bin,Xiaolong Shi
标识
DOI:10.1016/j.csbj.2024.02.021
摘要
Abstract
In recent years, DNA origami-based nanocarriers have been extensively utilized for efficient cancer therapy. However, developing a nanocarrier capable of effectively protecting cargos such as RNA remains a challenge. In this study, we designed a compact and controllable DNA tubular origami (DTO) measuring 120nm in length and 18nm in width. The DTO exhibited appropriate structural characteristics for encapsulating and safeguarding cargo. Inside the DTO, we incorporated 20 connecting points to facilitate the delivery of cargoes to various ovarian and normal epithelial cell lines. Specifically, fluorescent-labeled DNA strands were attached to these sites as cargoes. The DTO was engineered to open upon encountering miR-21 through RNA/DNA strand displacement. Significantly, for the first time, we inhibited fluorescence using the compact DNA nanotube and observed dynamic fluorescent signals, indicating the controllable opening of DTO through live-cell imaging. Our results demonstrated that the DTO remained properly closed, exhibited effective internalization in ovarian cancer cells in vitro, showcasing marked differential expression of miR-21, and efficiently opened with short-term exposure to miR-21. Leveraging its autonomous behavior and compact design, the DTO emerges as a promising nanocarrier for various clinically relevant materials. It holds significant application prospects in anti-cancer therapy and the development of flexible biosensors.
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