In vivo method to evaluate antioxidative activity using UVA‐induced carbonylated protein on human skin

氧化应激 抗氧化剂 活性氧 化学 体内 角鲨烯 角质层 人体皮肤 虾青素 脂质过氧化 食品科学 生物化学 药理学 类胡萝卜素 病理 生物 医学 生物技术 遗传学
作者
Changhui Cho,Eunyoung Lee,Eunbyul Cho,Heejoo Yoo,Jiyoun Bae,Jaehyoun Ha,Jae‐Sung Hwang
出处
期刊:Journal of Cosmetic Dermatology [Wiley]
卷期号:21 (3): 1263-1269 被引量:2
标识
DOI:10.1111/jocd.14227
摘要

Skin is continuously exposed to oxidative stress caused by reactive oxygen species (ROS) produced by the ultraviolet (UV) light, and it is important to evaluate the antioxidant activity. Carbonylated proteins (CPs) are candidate markers of oxidative modification as a result from the ROS. We aimed to develop the CP-based method to assess the efficacy of antioxidants in human skin.Ten healthy females were enrolled in the study to determine the UVA dosage for CP production, and another 10 females were included to evaluate the antioxidative activity. The stratum corneum was collected from test skin using D-Squame tape, and CPs from the SC were stained by fluorescence labeling and observed using a fluorescence microscope.CP level significantly increased with UVA irradiation from 15J/cm2 to 50J/cm2 compared to the control (non-UVA) area. CP production significantly increased by 34.38% and 35.22% in UVA irradiation and squalene (vehicle) areas. 5% α-tocopherol and β-carotene significantly increased the CP production by 20.77% and 19.34% after 2 hours of 30J of UVA irradiation compared to control area. Inhibition rate of CPs in 5% α-tocopherol and 5% β-carotene showed 41.45% and 45.37% after 2 hours of UVA irradiation.This study developed the simple, visual, and direct in vivo method to evaluate the antioxidative activity for products in human skin by measuring the CP level as an oxidative modification caused by UVA-induced ROS generation.
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