粘酸
儿茶酚
化学
产量(工程)
生物催化
水溶液
恶臭假单胞菌
有机化学
催化作用
酶
反应机理
苯
材料科学
冶金
作者
Aya Kaneko,Yoshitaka Ishii,Kohtaro Kirimura
出处
期刊:Chemistry Letters
[The Chemical Society of Japan]
日期:2011-03-12
卷期号:40 (4): 381-383
被引量:37
摘要
Abstract A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415 mM (59.0 g L−1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10 mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol−1).
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