斑马鱼
超氧化物歧化酶
氧化应激
生物
发育毒性
达尼奥
细胞凋亡
过氧化氢酶
胚胎
活性氧
谷胱甘肽
胚胎发生
虎耳草毒素
毒性
细胞生物学
DNA损伤
男科
分子生物学
生物化学
化学
遗传学
基因
毒素
胎儿
DNA
酶
医学
怀孕
有机化学
作者
Guoliang Chen,Zimu Jia,Linping Wang,Tingzhang Hu
标识
DOI:10.1016/j.envres.2020.109432
摘要
As a type of cyanobacterial toxins, saxitoxin (STX) is receiving great interest due to its increasing presence in waterbodies. However, the underlying mechanism of STX-induced adverse effect is poorly understood. Here, we examined the developmental toxicity and molecular mechanism induced by STX using zebrafish embryos as an animal model. The embryonic toxicity induced by STX was demonstrated by inhibition of embryo hatching, increase in mortality rate, abnormal heart rate, abnormalities in embryo morphology as well as defects in angiogenesis and common cardinal vein remodeling. STX induced embryonic DNA damage and cell apoptosis, which would be alleviated by antioxidant N-acetyl-L-cysteine. Additionally, STX significantly increased reactive oxygen species level, catalase activity and malondialdehyde content and decreased the activity of superoxide dismutase and glutathione content. STX also promoted the expression of vascular development-related genes DLL4 and VEGFC, and inhibited VEGFA expression. Furthermore, STX altered the transcriptional regulation of apoptosis-related genes (BAX, BCL-2, P53 and CASPASE 3). Taken together, STX induced adverse effect on development of zebrafish embryos, which might be associated with oxidative stress-induced apoptosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI