NLS公司
化学
转染
肽
半胱氨酸
脂质体
基因传递
阳离子脂质体
增强子
核定位序列
残留物(化学)
生物物理学
DNA
细胞毒性
环肽
生物化学
基因
细胞生物学
体外
基因表达
酶
生物
作者
Bieong-Kil Kim,Hyungu Kang,Kyung‐Oh Doh,Seong-Hye Lee,Jong‐Won Park,Sujin Lee,Tae-Jin Lee
标识
DOI:10.1016/j.bmcl.2012.07.051
摘要
Recently, cysteine residue incorporation increased liposome-mediated transfection compared to unmodified peptide. Therefore, we designed novel modified SV40 NLS peptides, homodimeric (NLS-CTHD, NLS-NTHD) and closed structure (cyclic NLS), simply using disulfide bond between cysteines to develop more efficient and safe non-viral gene delivery system. The simple mix of NLS-CTHD among these novel transfection enhancing peptides with DNA increased the gene transfer potency of cationic liposomes more efficiently with no additional cytotoxicity.
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